rabbit polyclonal igg against stat1  (Santa Cruz Biotechnology)

Journal: International Journal of Immunopathology and Pharmacology

Article Title: Activation of STAT6 by intranasal allergens correlated with the development of eosinophilic chronic rhinosinusitis in a mouse model

doi: 10.1177/03946320221109529

Figure Lengend Snippet: (a) Immunohistochemical staining for p-STAT1, p-STAT3, and p-STAT6. Marked p-STAT6 expression in the nucleus (stained brown-yellow or brown) (thin arrow) was observed in the mice treated with SEB + OVA compared with the mice treated with PBS, SEB, and OVA alone. (b–d) Quantitative analysis of p-STAT1-, p-STAT3-, and p-STAT6-positive cells. Groups C and D appeared to exhibit stronger positive signals for p-STAT6 than those of Groups A and B, and the numbers of p-STAT6-positive cells were significantly different (** p < 0.01). In addition, Group D showed the highest p-STAT6 expression (** p < 0.01). However, no significant differences in the numbers of p-STAT1-and p-STAT3-positive cells were observed among Groups A, B, C, and D. PBS indicates Group A; SEB indicates Group B; OVA indicates Group C; SEB + OVA indicates Group D. p-STAT6: phosphorylated STAT6.

Article Snippet: All sections were blocked with 1% normal goat serum after being washed with PBS and incubated for 12 h at 4°C with primary polyclonal antibodies against p-STAT1 (ET1611-20) (Huabio Ltd., Hangzhou, China), p-STAT3 (ET1607-39) (Huabio Ltd., Hangzhou, China), and p-STAT6 (ab28829) (Abcam Ltd., Cambridge, UK) according to the manufacturer’s instructions.

Techniques: Immunohistochemical staining, Staining, Expressing

Journal: International Journal of Immunopathology and Pharmacology

Article Title: Activation of STAT6 by intranasal allergens correlated with the development of eosinophilic chronic rhinosinusitis in a mouse model

doi: 10.1177/03946320221109529

Figure Lengend Snippet: The expression levels of the indicated proteins in the sinonasal mucosa were measured by western blotting. (a) Representative western blot images of STAT1, STAT3, STAT6, p-STAT1, p-STAT3, p-STAT6, T-bet, GATA-3, and RORγt. Protein expression of STAT1, STAT3, STAT6, p-STAT1, p-STAT3, p-STAT6, T-bet, GATA-3, and RORγt was quantified based on the density of the bands. GAPDH was used as a loading control. The relative expression levels of p-STAT1/STAT1 (b), p-STAT3/STAT3 (c), p-STAT6/STAT6 (d), T-bet/GAPDH (e), GATA-3/GAPDH (f), and RORγt/GAPDH (g) were calculated. Each column represents the mean ± SEM from five or six different animals, ** p < 0.01. PBS indicates Group A; SEB indicates Group B; OVA indicates Group C; SEB + OVA indicates Group D. OVA: ovalbumin; SEB: staphylococcal enterotoxin B.

Article Snippet: All sections were blocked with 1% normal goat serum after being washed with PBS and incubated for 12 h at 4°C with primary polyclonal antibodies against p-STAT1 (ET1611-20) (Huabio Ltd., Hangzhou, China), p-STAT3 (ET1607-39) (Huabio Ltd., Hangzhou, China), and p-STAT6 (ab28829) (Abcam Ltd., Cambridge, UK) according to the manufacturer’s instructions.

Techniques: Expressing, Western Blot, Control